Totipotent stem cells are able to divide and differentiate into cells of the whole organism. This chapter will focus on stem cell classification to make it easier for the reader to comprehend the following chapters. Developmental potency is reduced with each step, which means that a unipotent stem cell is not able to differentiate into as many types of cells as a pluripotent one. There are several steps of specialization. Stem cells exist both in embryos and adult cells. They are able to differentiate into any cell of an organism and have the ability of self-renewal. Stem cells are unspecialized cells of the human body. A wide variety of possibilities makes this cutting edge therapy a turning point in modern medicine, providing hope for untreatable diseases.
The review is summarized by challenges that stem cell therapy must overcome to be accepted worldwide. Among many types of stem tissue applications, the use of graphene scaffolds and the potential of extracellular vesicle-based therapies require attention due to their versatility. Derivation methods and the utilization of culturing media are crucial to set proper environmental conditions for controlled differentiation. Quality control and teratoma formation assays are important procedures in assessing the properties of the stem cells tested. The genesis of stem cells is followed by laboratory steps of controlled stem cell culturing and derivation. This paper is a review focused on the discovery of different stem cells and the potential therapies based on these cells. The development of treatment methods has evoked great expectations. In recent years, stem cell therapy has become a very promising and advanced scientific research topic.
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